Kit for the serological diagnosis of Newcastle Disease
- Antigen: stabilized and freeze-dried Newcastle Disease Virus (NDV), La Sota strain.
- Positive serum: hyper immune serum against Newcastle Disease obtained from SPF fowls
- Negative serum: serum obtained from SPF fowls (no antibodies against NDV)
- Diluent: phosphate buffer saline solution (PBS) with pH 7,2 – 7,4 ± 0,15%
Chickens, turkeys, guinea fowls, pheasants, quails, pigeons
The Nd-Test is used for diagnosis and to evaluate the degree of humoral immunity by inhibohemagglutination reaction in fowls vaccinated against Newcastle disease (avian pseudopest).
METHOD OF USE AND RESULT INTERPRETATION
Reagent verification and IHA reaction are described in the leaflet.
Interpretation of results
According to the OIE standards, the test is positive if there is a hemagglutination inhibition to minimum 1/16 testing serum dilution, using 4-UHA antigen. Monitor the immune status of fowls vaccinated against Newcastle Disease (ND) 14 days after the last vaccination in case of live vaccines and 21 days after inactive vaccines. Based on laboratory observations and epidemiological surveillance carried out to evaluate the immune status using the Nd-Test, we consider the following:
- After the first vaccination against ND, 75% of the tested samples must have titers between 1/16 (24) – 1/128 (27); (these titers are obtained if the vaccines are applied by oculo/nasal route, spray or aerosols); if the vaccines are administered via drinking water or injection, the titers are a dilution smaller;
- In flocks with 2-4 vaccinations against ND, 75% of the tested samples must have titers between 1/128 (27) – 1/256 (28);
In flocks with more than 5 vaccinations, including the inactivated vaccines, 75% of the tested samples must have titers between 1/512 (29) – 1/2048 (211). If titers higher than 1/4096 (212) are detected, carry out the epidemiological surveillance and viral examination in the laboratory.
ADVICE ON CORRECT USE
Store the kit at 2–8 °C protected from light. After rehydration and first use, divide in small amounts and store at -20 °C, avoiding repeated freezing and thawing which could influence the results. Do not use expired reagents or components from different kits.
The kit contains:
|ml||Number of tests|
|Freeze-dried antigen||1||50, 100, 200, 400|
|Freeze-dried positive serum||1||50, 100, 200, 400|
|Freeze-dried negative serum||5||50, 100, 200, 400|
|Diluent||7||50, 100, 200, 400|